منابع مشابه
Structure analysis of microRNA precursors.
MicroRNA biogenesis occurs in several steps from their precursors having irregular hairpin structures. The highly variable architecture of these stem-and-loop structures, which have terminal loops of various sizes and diverse structure destabilizing motifs present in their stem sections, may strongly influence the process of microRNA liberation. In order to better understand this process, more ...
متن کاملNuclear export of microRNA precursors.
MicroRNAs (miRNAs), which function as regulators of gene expression in eukaryotes, are processed from larger transcripts by sequential action of nuclear and cytoplasmic ribonuclease III-like endonucleases. We show that Exportin-5 (Exp5) mediates efficient nuclear export of short miRNA precursors (pre-miRNAs) and that its depletion by RNA interference results in reduced miRNA levels. Exp5 binds ...
متن کاملMammalian 5′-Capped MicroRNA Precursors that Generate a Single MicroRNA
MicroRNAs (miRNAs) are short RNA gene regulators typically produced from primary transcripts that are cleaved by the nuclear microprocessor complex, with the resulting precursor miRNA hairpins exported by exportin 5 and processed by cytoplasmic Dicer to yield two (5p and 3p) miRNAs. Here, we document microprocessor-independent 7-methylguanosine (m(7)G)-capped pre-miRNAs, whose 5' ends coincide ...
متن کاملRegulation of microRNA-mediated gene silencing by microRNA precursors
miRNAs are a class of about 1,000 noncoding RNAs that in mammals regulate about half of the protein-encoding genes by enhancing their degradation or preventing their translation1,2. After transcription, the long primary miRNA (pri-miRNA) transcripts are processed to 60to 80-nt precursor miRNAs (pre-miRNAs) and are subsequently cleaved to mature ~22-nt-long duplex RNAs3,4. With a few exceptions5...
متن کاملIdentification of MicroRNA Precursors via SVM
MiRNAs are short non-coding RNAs that regulate gene expression. While the first miRNAs were discovered using experimental methods, experimental miRNA identification remains technically challenging and incomplete. This calls for the development of computational approaches to complement experimental approaches to miRNA gene identification. We propose in this paper a de novo miRNA precursor predic...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2006
ISSN: 1471-2164
DOI: 10.1186/1471-2164-7-285